Toxicity Determination Of Insecticide

1. Spray method: The spray method designed to simulate the actual prevention and control of forests. In order to reduce the test error, each test insect should receive the same dose as much as possible, and the sprayer should have a certain pressure to make the size of the fog point consistent. Spray evenly.

2. Liquid immersion method: the test insects are separately immersed in different concentrations of the diluted liquid, immediately removed, placed on the absorbent paper to absorb too much liquid, transferred into each group of containers, given fresh The leaves are used as feed, and the gauze is covered with gauze or gauze. It is kept indoors, room temperature is recorded, and the death of poisoning at different times is observed.

3. Membrane method: Spray the agent on a certain area of the surface to form a uniform film (usually a petri dish with filter paper, or take the needles in the liquid to be taken out), and then let The tested pests are on the top, and after being in contact with the drug film for a certain period of time, they are returned to the normal container for feeding, or during the process of contacting the drug film, the poisoning death is observed.

4. Drip method: using a quantitative amount of liquid droplets, drip on the back of the chest of the test insects, and then regularly observe the death of poisoning. The advantage of this method is that the dose can be expressed by the amount of drug received per gram of worm (usually used in micrograms), the dosage is easy to control, and the test error is small; the disadvantage is time-consuming, the solvent used for the treatment, the location of the droplets, and the size of the droplets, etc. Can affect virulence. In adopting this law, these factors should be considered and agreed as much as possible in order to obtain accurate results.